My thesis

To come back on my thesis, the last post to tell you how far the situation is.

So there were three main goals of my thesis:

1. Investigate the dissolution characteristics of different CaP scaffolds

2. Investigate the cell seeding efficiency of the different scaffold types

3. Investigate the gene expression of cultured cells on the different scaffolds

To start, the in vivo used scaffolds were analyzed with nanoCT first to investigate the structural characteristics of the scaffolds. Together with the material characteristics we, in the end, try to correlate this to the results of the different parts.

Since bone formation needs osteoconduction, -induction and -genesis, we investigate the dissolution of the scaffolds for the decomposition of Calcium and Phosphate ions as an inductive path for the cells to stimulate differentiation to osteoblasts. The conductive way of stimulating the cells is investigated through material and structural information on the scaffold. Osteogenesis or bone formation by cells can be assessed in vitro by doing RNA extraction of the cultured cells and to quantify certain gene markers. If certain genes like RUNX2, OCN, Col1A1 are overrepresented (compared to housekeeping genes) we can gently predict that the scaffold in vivo will stimulate bone. This can then be correlated with the dissolution characteristics, cell and scaffold properties to make up an overall discussion, based on other research results found in literature.

To tell you how far I am. The lab work of steps 1 and 2 are finished but the results are being investigated. Step 3 will-be started this thursday and the lab work will be done after 5 weeks. Then still the results will have to be investigated.

I hope you have a clear picture of what my thesis is. The results I cannot show but when my thesis is finished you can always ask me for the results if you would like to know.

Thank you for your time 🙂

The animal discussion will be continued for a few more weeks.

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  1. 13/03/2013 at 16:31

    This sounds very interesting!

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