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Scaffold analysis

Back to my thesis…

As I told you before, a scaffold is a carrier wherein cells can attach themselves and this cellularized scaffold can then be implanted to regenerate more efficiently new tissue. The scaffolds we are using are biodegradable, made from CaP and some have a collagen matrix distributed between the CaP grains.

Bio-Oss-Collagen.6692106_std

An important issue is the search after the optimal morphology of a scaffold (apart from the material type). This includes some parameters as average pore size, total void volume, porosity, specific surface area, etc. The difficulty here is that a scaffold is small and cored out of a larger block, delivered by the manufacturer. Structural properties between different blocks are quite homogenous, but between different scaffolds there are quite some variations between these structural parameters. Of course analyzing these scaffolds is the solution, but this takes an enormous amount of time (scanning and computing thousands of pictures).

A possible way to calculate these structural parameters is micro-CT analysis. It takes around 2000 pictures. These pictures are made from the scatter that x-ray beams of different positions (angle, height, …) experience through the sample. Computer software then can calculate 3D parameters. The outcome of the first 8 samples of each scaffold type resulted in next graphs:

1: Total scaffold volume 2: Total void volume

1: Total scaffold volume
2: Total void volume

1: Porosity 2: SSA

1: Porosity
2: SSA

As you can see it is very difficult to cut every scaffold manually with the same size. This is a necessity since it will be important to have scaffolds of the same size in further experiments. The intrinsic parameters are independent of size and though very only between the different scaffold types.

So now you guys are also up to date! 🙂

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  1. xieaizhi
    07/03/2013 at 13:41

    Honestly, I feel uncomfortable when I see the first picture.
    But, I would be nice if we can find a right size of scaffold to help people fixing our body.

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